Publications during undergraduate research:
An optimized acetylcholine sensor for monitoring in vivo cholinergic activity, Nature Methods, 2020
Authors: Jing, M., Li, Y.*, Zeng, J., … Li, Y.
A Genetically Encoded Protein Polymer for Uranyl Binding and Extraction Based on the SpyTag–SpyCatcher Chemistry, ACS Synth. Biol., 2018
Authors: Yang, X., Wei, J., …, Li, Y.*, … Qian, L.
Activation of the anti-aging and cognition-enhancing gene klotho by CRISPR-dCas9 transcriptional effector complex, J Molec Neurosci, 2018
Authors: Chen C.Di, Zeldich E, Li, Y.*, … Abraham, C. R.
Identification of the cleavage sites leading to the shed forms of human and mouse anti-aging and cognition-enhancing protein Klotho, PLoS ONE, 2020
Authors: Chen, C. Di, Li, Y.*, … Abraham, C. R.
Research experiences behind the publication
GACh sensors
This work was done during my undergraduate thesis research with Professor Yulong Li at Peking University in 2017. My goal was to optimize the performance of the origanl GACh (Genetically encoded Acetylcholine) sensor. With structure-directed mutagenesis and cell-based optical screening, I found an improved version of GACh sensors (GACh 3.0). With collaborations with other labs, we further characterized its properties in multiple preparations. GACh sensor is widely used in the neuroscience community and leading to new discoveries.
Expression of GACh 3.0 in cultured neurons
Uranium Reaper
I was a team member of iGEM (international Genetically Engineered Machine) representing Peking University in 2016. We constructed a novel functional biomaterial consisting of multiple functional protein modules. This material is designed to be produced and secreted by bacteria, and self-assembled to form a polymer network. In combination with a specific Super Uranyl-binding Protein, it obtains the ability to adsorb uranyl ions. After very short contacting with polluted water, the uranyl-laden biomaterial, which also contains a monomeric streptavidin module, could be easily cleared using biotinylated magnetic beads. Our work was awarded Best Environment prize.
This experience brought me the joy of doing research, coming up with ideas, designing and performing experiments, and discussing the results keep me motiviated. Working with a team also inspired me a lot, the team spirit we held to complete the project and to present our work at the competition really supported and strengthed me.
Design of the Uranium Reaper
Klotho
Kloth plays a crucial role in human health. It serves as a powerful regulator of aging and numerous physiological processes. Klotho infuences kidney function, insulin sensitivity, antioxidant activity, and cellular stress responses, all of which are pivotal for longevity. Its deficiency is linked to various age-related diseases, such as cardiovascular issues and neurodegenerative disorders. Understanding and harnessing Klotho’s potential may hold the key to extending healthy lifespans and combating age-related ailments. During my summer research at Boston Universiyt, I identified the cleavage site of mouse Klotho. Moreover, I applied CRISPR-dCas9 activation system to enhance the transcription of klotho gene. These results contribute to our understanding of Klotho.